论文部分内容阅读
磷是植物生长的必需元素之一,对植物生长发育、生理代谢、产量与品质形成起着十分重要作用。研究耐低磷植物,有助于提高作物产量,降低化肥用量,提高化肥利用率。本研究通过在不同供磷条件下对转OsPup1基因植株的形态进行研究,阐明该基因在不同供磷水平对植株生长特性的影响。通过农杆菌介导法将OsPup1基因遗传转化烟草,经抗性筛选和PCR鉴定获得转基因植株。对不同转基因烟草株系,设置4个磷水平处理进行耐磷性及铝毒性分析。结果显示,转OsPup1基因烟草以0.25 mmol/L KH2PO4作为低磷处理较适合,与野生型植株相比,转基因株系植株根系数量增多,主侧根增加。在低磷胁迫及三氯化铝(Al Cl3)处理条件下转OsPup1基因烟草的生长情况明显优于野生型植株。说明转OsPup1基因对环境中的低磷胁迫逆境能产生明显应答,初步推断OsPup1基因的表达,具有增加植株根系,改善植株生长状态和延缓铝毒的生物学功能。本研究表明,OsPup1基因在介导植株不同供磷水平下的根叶形态建成发挥着重要作用。
Phosphorus is one of the essential elements of plant growth and plays an important role in plant growth and development, physiological metabolism, yield and quality formation. Research on low-phosphorus-tolerant plants will help increase crop yield, reduce fertilizer use and increase fertilizer use efficiency. In this study, the morphological changes of OsPup1 transgenic plants were studied under different P supplying conditions to elucidate the effects of different gene expression levels on the plant growth characteristics. The OsPup1 gene was transformed into tobacco by Agrobacterium tumefaciens-mediated method. The transgenic plants were obtained by resistance screening and PCR identification. For different transgenic tobacco lines, four phosphorus levels were set for phosphorus tolerance and aluminum toxicity analysis. The results showed that transgenic plants treated with 0.25 mmol / L KH2PO4 were suitable for low-phosphorus treatment. Compared with wild-type plants, the number of transgenic plants increased and the number of major lateral roots increased. Under low phosphorus stress and aluminum chloride (Al Cl3) treatment under the conditions of OsPup1 transgenic tobacco growth was significantly better than the wild-type plants. The results showed that OsPup1 gene could produce significant response to environmental stresses of low P stress, and preliminarily deduced the expression of OsPup1 gene, which could increase the plant root system, improve plant growth status and delay the biological function of Al toxicity. The present study shows that OsPup1 plays an important role in the formation of root and shoot under different levels of phosphorus supply.