论文部分内容阅读
目的探讨豚鼠哮喘模型 Th_1/Th_2细胞亚群的平衡及其意义。方法用酶联免疫吸附法测定BALF 中 Th_1细胞因子 IFN-γ、Th_2细胞因子 IL-4浓度及其比值,并进行组间比较。结果本实验采用卵蛋白(OVA)腹腔注射致敏加雾化吸入激发的方法复制豚鼠哮喘模型,动物都表现有不同程度的过敏反应症状。实验动物支气管肺泡灌洗液(BALF)中炎症细胞的变化:嗜酸性粒细胞(EOS)个数 A 组、B 组分别为(98±58)×10~6/L、(12±10)×10~6/L,A 组与 B 组比较差异有统计学意义(P<0.01)。Th_1相关细胞因子IFN-γ浓度 A 组明显低于 B 组,差异有统计学意义(P<0.05);Th_2相关细胞因子 IL-4浓度 A 组明显高于B组,差异有统计学意义(P<0.05);IFN-γ/IL-4比值 A 组相应低于 B 组。结论豚鼠哮喘模型中 Th_1/Th_2细胞亚群发生失衡,维持 Th_1/Th_2细胞亚群平衡对哮喘防治具有积极的意义。
Objective To investigate the balance of Th1 / Th2 cell subsets in guinea pig asthma model and its significance. Methods Th1 cytokine IFN-γ, Th2 cytokine IL-4 concentration in BALF were measured by enzyme-linked immunosorbent assay (ELISA) and their ratios were compared between groups. Results In this experiment, guinea pig asthma model was reproduced by intraperitoneal injection of ovalbumin (OVA) and sensitized by inhalation by atomization. All animals showed symptoms of allergic reaction to varying degrees. The changes of inflammatory cells in bronchoalveolar lavage fluid (BALF) of experimental animals were as follows: the number of eosinophils (EOS) in group A was (98 ± 58) × 10 ~ 6 / L, (12 ± 10) × 10 ~ 6 / L, the difference between group A and group B was statistically significant (P <0.01). Th1-related cytokine IFN-γ concentration in group A was significantly lower than that in group B, the difference was statistically significant (P <0.05); Th2-related cytokines IL-4 concentration in group A was significantly higher than group B, the difference was statistically significant <0.05). The IFN-γ / IL-4 ratio in group A was lower than that in group B respectively. Conclusions The imbalance of Th1 / Th2 cell subsets in asthmatic guinea pigs and the balance of Th1 / Th2 cell subsets have a positive effect on the prevention and treatment of asthma.