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目的探讨纳米炭注射液用于肝癌淋巴结示踪的价值。方法 2只健康日本大白兔肌内注射VX2细胞株制备荷瘤兔,切除肿瘤后将1mm×1mm×1mm瘤块开腹种植于30只日本大白兔腹腔,制备兔肝癌模型。将30只日本大白兔随机分为对照组、亚甲蓝组与纳米炭组各10只,3组均切除肝癌病灶,清扫局部淋巴结,并于试验结束处死动物,观察肝脏周围淋巴结转移癌数目、示踪剂对淋巴结转移癌的示踪效果。结果对照组、亚甲蓝组和纳米炭组切除淋巴结数目分别为21、14、17个,差异无统计学意义(P>0.05);对照组、亚甲蓝组、纳米炭组对兔肝癌淋巴结示踪的敏感性分别为47.6%、84.2%、87.5%(P<0.05);特异性分别为40.91%、88.89%、86.96%(P<0.01);纳米炭组淋巴结染色时间(100.50±29.92)s较亚甲蓝组(11.20±4.18)s长(P<0.01);纳米炭组淋巴结褪色时间(2.22±0.74)h与亚甲蓝组(1.63±0.54)h比较差异无统计学意义(P>0.05)。结论兔肝癌模型行肝癌病灶切除和淋巴结清扫手术中应用纳米炭注射液对肝癌及淋巴引流有明显示踪作用。
Objective To investigate the value of nano-carbon injection for liver cancer lymph node tracing. Methods 2 healthy Japanese white rabbits were injected intramuscularly with VX2 cell line to prepare tumor-bearing rabbits. After tumor resection, a 1mm × 1mm × 1mm tumor mass was implanted in the abdominal cavity of 30 Japanese white rabbits to prepare rabbit liver cancer model. The 30 Japanese white rabbits were randomly divided into control group, group and methylene blue carbon nano groups of 10, three groups were excised liver lesions, local lymph node dissection, and the animals were sacrificed at the end of the test, the number of lymph node metastasis was observed around the hepatic cancer, Tracer effect on lymph node metastasis. Results The number of resected lymph nodes in control group, methylene blue group and nanocarbon group was 21, 14 and 17, respectively, with no significant difference (P> 0.05). The control group, methylene blue group and nanocarbon group The sensitivity of tracing was 47.6%, 84.2% and 87.5% respectively (P <0.05), and the specificity was 40.91%, 88.89% and 86.96% respectively (P <0.01) s was longer than that of methylene blue (11.20 ± 4.18) s (P <0.01). There was no significant difference in the fade time of lymph nodes between nano-charcoal (2.22 ± 0.74) h and methylene blue > 0.05). Conclusion The application of nano-carbon injection in hepatic carcinoma and lymphatic drainage in rabbit liver cancer model resection and lymph node dissection have obvious tracing effect.