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强化生物除磷是废水生物除磷的主要技术手段。在厌氧与好氧交替运行的反应过程中,污泥中聚β羟基烷酸酯(PHA)经历了厌氧储存和好氧降解的过程,其位于1 740cm-1吸收峰亦呈现出先增强后减弱的变化。通过比较PHA标准品的红外光谱图,实现了活性污泥PHA的红外特征归属,1 740cm-1红外吸收峰源于PHA羰基特征吸收。利用高斯分峰法将相互重叠的PHA峰、蛋白质Ⅰ峰和蛋白质Ⅱ峰进行了分离,PHA与蛋白质Ⅰ峰的峰面积比值与PHA测定值有较好相关性,相关系数可达0.873,峰面积比值可反映污泥中PHA的变化趋势。选取1 480~1 780cm-1区域红外光谱,经过归一化处理并转化为吸光度数据后,结合污泥样品PHA含量的色谱分析结果,应用偏最小二乘法建立了污泥样品红外光谱与PHA含量的关系模型,模型预测值与测量值具有较好的一致性,结合未知浓度活性污泥样品的红外光谱以及该关系模型,可以迅速预测未知污泥样品中的PHA含量。该方法的研究为污泥胞内PHA的快速表征和定量分析提供了新的分析方法。
Strengthen the biological phosphorus removal of biological phosphorus removal of the main technical means. During the reaction process of anaerobic and aerobic alternation, poly (β-hydroxyalkanoate) (PHA) in sludge undergoes the process of anaerobic storage and aerobic degradation. The absorption peak at 1 740 cm-1 also shows the first increase Weakened change. By comparing the infrared spectra of PHA standard, the infrared characteristics of activated sludge PHA were obtained. The infrared absorption peak at 1 740cm-1 originated from the absorption of PHA carbonyl. The peak of PHA peak, protein Ⅰ peak and protein Ⅱ peak were separated by Gaussian peak-splitting method. The ratio of peak area of PHA to peak I of protein was well correlated with the value of PHA. The correlation coefficient was 0.873. The peak area The ratio reflects the trend of PHA in sludge. The infrared spectrum of 1 480 ~ 1 780cm-1 region was selected, and after normalized and converted into absorbance data, combined with the chromatographic analysis results of PHA content in sludge samples, partial least square method was used to establish the infrared spectra and PHA content The relationship between model predictive value and measured value has good consistency. Combined with the infrared spectra of unknown concentration activated sludge samples and the relationship model, the PHA content of unknown sludge samples can be rapidly predicted. This method provides a new analytical method for the rapid characterization and quantitative analysis of PHA in sludge.