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目的对2010年邢台市手足口病重症病例进行病原体检测。方法收集临床诊断为手足口病重症病例的疱疹液、咽拭子和肛拭子标本385份,采用实时荧光定量PCR对这些标本进行人肠道病毒71型、柯萨奇病毒A组16型和其它肠道病毒的分型检测。结果人肠道病毒71型阳性率80.00%,柯萨奇病毒A组16型阳性率0.26%,其它肠道病毒阳性率7.53%;阳性率较高的地区为东南部平原人口密集区,发病率较高的年龄组为1～3岁,男性多于女性。结论实时荧光定量PCR可用于手足口病的病原体分型检测,一旦发现手足口病患者感染的病原体为人肠道病毒71型,可提示临床医生警惕手足口病重症病例的发生,对减少手足口病重症病例发生及防止患儿死亡具有重要作用。
Objective To detect pathogens in severe cases of hand, foot and mouth disease in Xingtai City in 2010. Methods 385 samples of herpes fluid, throat swab and anal swab clinically diagnosed as hand-foot-mouth disease were collected. The specimens were tested for human enterovirus 71, Coxsackie virus A group 16 and real-time fluorescent quantitative PCR Typing of other enteroviruses. Results The positive rate of human enterovirus 71 was 80.00%, the positive rate of Coxsackievirus A 16 was 0.26%, and the positive rate of other enterovirus was 7.53%. The positive rate was found in the southeastern plain with dense population, The higher age group is 1 to 3 years old, more men than women. Conclusion Real-time PCR can be used to detect the pathogens of hand, foot and mouth disease. Once the pathogens of hand, foot and mouth disease are found to be human enterovirus 71, it may prompt clinicians to guard against the occurrence of severe cases of HFMD. Occurrence of severe cases and prevent the death of children has an important role.