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目的:比较荧光偏振免疫分析法(FPIA)和酶增强免疫法(EMIT)监测甲氨蝶呤(MTX)血浆药物浓度的相关性。方法:收集接受大剂量甲氨蝶呤化疗后的患儿血液样品,分别用FPIA和EMIT法进行测定,考察2种测定方法的相关程度。结果:在MTX血浆浓度≥0.30μmol·L-1时,以FPIA法测定结果(X)和EMIT法测定结果(Y)所作的线性回归方程如下:Y=1.0451 X+0.1153,两组结果间存在较好的相关性(Spearmen相关系数为0.968),EMIT方法的测定结果高于FPIA法所测结果,差异有统计学意义(P<0.05)。结论:MTX血浆药物浓度≥0.30μmol·L-1时,EMIT法测定结果高于FPIA法,且和后者相关性良好,经相互换算后可用于指导四氢叶酸钙(CF)的解救。
OBJECTIVE: To compare the plasma concentrations of methotrexate (MTX) with fluorescence polarization immunoassay (FPIA) and enzyme immunoassay (EMIT). Methods: Blood samples of children received high dose methotrexate chemotherapy were collected and measured by FPIA and EMIT respectively. The correlation between the two methods was investigated. Results: The linear regression equation determined by the FPIA assay (X) and the EMIT assay (Y) at MTX plasma concentration ≥0.30 μmol·L-1 was as follows: Y = 1.0451 X + 0.1153, (Spearmen correlation coefficient was 0.968). The results of EMIT method were higher than those of FPIA method, the difference was statistically significant (P <0.05). CONCLUSION: MTX plasma drug concentration ≥0.30μmol·L-1, EMIT determination results higher than the FPIA method, and the correlation between the good, after conversion can be used to guide the rescue of calcium hydrate (CF).