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本文应用生长抑素、5-羟色胺、P物质,胶质酸性蛋白的抗体,通过免疫细胞化学反应,对胚胎脊髓组织移植后的细胞构筑特征进行了观察。结果发现,移植后2—6个月的胚胎脊髓组织内分布有与上述抗体相应的免疫阳性神经元及神经纤维。在移植组织与宿主脊髓融合差的情况下,移植界面可见胶质酸性蛋白免疫反应明显增强。研究表明,免疫细胞化学方法可反映移植组织的生长分化程度,并可显示移植部位的细胞构筑特征。
In this paper, we used the somatostatin, serotonin, substance P and glial acidic protein antibodies to observe the characteristics of cell construction after embryo spinal cord transplantation by immunocytochemistry. The results showed that immune positive neurons and nerve fibers corresponding to the above antibodies were distributed in embryonic spinal cord tissue 2-6 months after transplantation. In the case of poor graft and host spinal fusion, glial acidic protein immunoreactivity was significantly enhanced at the graft interface. Studies have shown that immunocytochemistry can reflect the degree of growth and differentiation of the transplanted tissue and can display the cell construction features of the transplanted site.