应用反相高效液相色谱法，建立了测定纯中药制剂补肾益寿颗粒中活性成分淫羊藿甙含量的方法．样品以７０％乙醇超声波振荡提取，在下列色谱条件下测定：固定相ｓｈｉｍｐａｃｋＣＬＣ－ＯＤＳ（１５０ｍｍ×６ｍｍ，５μｍ）；流动相乙腈甲醇－０．１ｍｏｌ／Ｌ磷酸二氢钠（６∶４６∶５０，ｖ／ｖ）；流速１．０ｍｌ／ｍｉｎ；检测波长２７０ｎｍ；线性范围０．１１５～０．６９０μｇ（ｒ＝０．９９９９）；加样回收率９９．０８±１．７６％；日内精密度１．０５％～１．１９％．该法简便、快速、准确 The method of determination of the content of epimedium, the active ingredient in the pure Chinese medicine preparation Bushen Yishou Granules, was established by reversed-phase high performance liquid chromatography. The sample was extracted by ultrasonic shaking with 70% ethanol, and determined under the following chromatographic conditions: stationary phase shim-packCLC-ODS (150 mm×6 mm, 5 μm); mobile phase acetonitrile-methanol-0.1 mol/L sodium dihydrogen phosphate (6:46 : 50, v/v); flow rate 1.0 ml/min; detection wavelength 270 nm; linear range 0.115 to 0.690 μg (r=0.9999); sample recovery rate 99.08±1.76%; days Precision 1.05% to 1.19%. The method is simple, fast and accurate