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目的 了解江苏淮阴地区单采浆献血员丙型肝炎病毒 (HCV)感染后发病情况和病毒基因分型。方法应用ELISA法及RT -nestedPCR法检测 6 7例单采浆献血员感染HCV后抗 -HCV和HCVRNA ,并用限制性片段长度多态性分析法 (RFLP)进行病毒基因分型。结果 6 7例单采浆献血员 ,在感染HCV后 ,急性丙型肝炎发病率为 17.9% (12 / 6 7) ,最终发展成慢性丙型肝炎者 49.3% (33/ 6 7) ,HCV慢性持续感染 6 8.8% (4 6 / 6 7)。HCV基因分型 :1b/Ⅱ型占 95 .5 % (6 4/ 6 7) ,2a/Ⅲ型 1.5 % (1/ 6 7) ,1b/ 2a混合型 3.0 % (2 / 6 7)。结论 淮阴地区单采浆献血员HCV感染以 1b/Ⅱ型为优势株 ,慢性化比例高
Objective To understand the incidence and genotyping of hepatitis C virus (HCV) infection in apheresis blood donors in Huaiyin area of Jiangsu province. Methods Anti-HCV and HCV RNA were detected by ELISA and RT -nestedPCR in 67 post single-donor blood donors infected with HCV, and genotyped by restriction fragment length polymorphism (RFLP). RESULTS: Sixty-seven single-plasmapheresis donors had an HCV infection rate of 17.9% (12/67) and 49.3% (33/67) of those who developed chronic hepatitis C infection. Chronic hepatitis C Continuous infection was 6 8.8% (46/67). The genotypes of HCV genotype 1b / Ⅱ accounted for 95.5% (64/67), 2a / Ⅲ 1.5% (1/67), 1b / 2a mixed 3.0% (2/67). Conclusion The HCV infection of apheresis blood donors in Huaiyin area is predominant in type 1b / Ⅱ, with a high proportion of chronicity