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在玻碳电极上电沉积石墨烯/壳聚糖/碳纳米管复合膜,通过戊二醛连接抗体,建立了新型电流型甲胎蛋白传感器,通过循环伏安法和交流阻抗考察了其电化学特性。在优化的实验条件下,传感器的峰电流随着检测溶液中甲胎蛋白(AFP)浓度的增大而增大,并在0.05～100ng/mL浓度范围内呈现线性关系,回归方程为△I=0.51c+0.68(ng/mL);检测限为0.02ng/mL(R=0.9990)。该免疫传感器具有制作简单、灵敏度较高、重现性好、线性范围宽等优点,可用于临床上对AFP的检测。
A novel current-type alpha-fetoprotein sensor was established by electrodepositing graphene / chitosan / carbon nanotube composite membrane on glassy carbon electrode by glutaraldehyde antibody. The electrochemical properties of the current-type alpha-fetoprotein sensor were investigated by cyclic voltammetry and electrochemical impedance spectroscopy characteristic. Under optimized experimental conditions, the peak current of the sensor increased with the increase of the concentration of AFP in the test solution and showed a linear relationship in the concentration range of 0.05-100 ng / mL. The regression equation was ΔI = 0.51c + 0.68 (ng / mL); detection limit was 0.02 ng / mL (R = 0.9990). The immunosensor has the advantages of simple manufacture, high sensitivity, good reproducibility and wide linear range, and can be used for the clinical detection of AFP.