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目的 在染色体水平对决定果蝇七氟醚敏感性的基因进行定位。方法 将H(黑腹 )处女雌蝇与e(黑檀体 )雄蝇进行杂交产生杂合子一代 (F1)。取F1雄蝇与eF1处女雌蝇进行侧交产生子二代 (F2 )。按性别及表型分别测定三代果蝇七氟醚麻醉的ED50 值 ,比较侧交F2 中黑腹、黑檀体表型果蝇和H、e自交F2 果蝇的ED50 值 ,并作浓度 -效应曲线。结果 杂合F1ED50 与HF1ED50 相等 ,大于eF1的ED50 值。侧交F2 中 49.76 %表现为黑腹 ,5 0 .2 4%表现为黑檀体。F2 中黑腹和黑檀体ED50值大于eF2 的ED50 ,小于HF2 的ED50 值 ,且其量 -效曲线均为双峰组成 ,而H和e自交F2 的量 -效曲线均为单峰。结论 决定黑腹果蝇对七氟醚敏感性的基因位于果蝇第二号染色体。
Objective To locate the genes responsible for sevoflurane sensitivity in Drosophila at chromosome level. Methods H (Black Belly) virgin female flies and e (Ebony) male flies were crossed to produce a heterozygous generation (F1). F1 male flies and eF1 virgin female flies were taken to produce the second generation (F2). The ED50 values of sesame seeds of three generation Drosophila sevoflurane were determined by sex and phenotype. The ED50 values of Drosophila melanogaster, Diantle Ebony, Drosophila melanogaster and H, e self F2 flies were compared and the concentration- Effect curve. Results Hybrid F1ED50 was equal to HF1ED50, greater than the ED50 value of eF1. 49.76% of lateral cross F2 showed black stomach, 50.24% showed ebony body. The ED50 value of F2 and F2 was greater than that of eF2, which was smaller than that of HF2, and its quantity-response curve was bimodal, while the quantity-response curves of F2 and F2 were both single peak. Conclusion The gene that determines the sensitivity of Drosophila melanogaster to sevoflurane is located on the second chromosome of Drosophila.