论文部分内容阅读
目的:探讨贵州省思南县汉族与苗族、土家族女性5,10-亚甲基四氢叶酸还原酶(MTHFR)C677T、A1298C及甲硫氨酸合成酶还原酶(MTRR)A66G基因多态性的频率特征。方法:以2013年12月~2014年12月思南县1232例汉族、苗族、土家族女性为研究对象,采集口腔黏膜上皮细胞,提取DNA,采用荧光定量PCR,进行编码基因MTHFR和MTRR的相关多态性位点检测。结果:思南县汉族与土家族女性的MTHFR C677T基因型频率差异有统计学意义(X~2=7.53,P<0.05)。汉族、苗族、土家族女性MTHFR A1298C和MTRR A66G等位基因频率差异无统计学意义。结论:思南县汉族女性MTHFR 677TT基因型的频率高于土家族女性。对叶酸代谢障碍遗传检测有风险的人群增加叶酸补服的剂量与时间,可达到一级预防的目的。
Objective: To investigate the polymorphism of MTHFR C677T, A1298C and methionine synthase reductase (MTRR) A66G in Han and Miao nationality and Tujia ethnic group in Sinan County, Guizhou Province. Frequency characteristics. METHODS: A total of 1232 Han, Miao and Tujia women in Sinan County from December 2013 to December 2014 were enrolled in this study. Oral mucosal epithelial cells were collected and DNA extracted. Fluorescent quantitative PCR was used to detect the correlation between MTHFR and MTRR Polymorphic site detection. Results: The frequencies of MTHFR C677T genotype in Sinan Han and Tujia women were statistically different (X ~ 2 = 7.53, P <0.05). Han, Miao and Tujia women MTHFR A1298C and MTRR A66G allele frequency was no significant difference. Conclusion: The frequency of MTHFR 677TT genotype in Han nationality in Sinan County is higher than that in Tujia women. Folic acid metabolism risk of genetic testing in people at increased doses of folic acid supplementation and time, can achieve the purpose of primary prevention.