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将具有分子识别功能的乙酰胆碱酯酶和胆碱氧化酶及进行换能反应的luminol和Cu~(2+)分别固定在多孔玻璃和离子交换剂的柱中,组成流动注射式胆碱和乙酰胆碱化学发光传感器,让传感器分子识别反应和换能反应在各自最佳pH值下进行.这一新型生物传感器优化了发光量子产率,避免了在传感元件上直接发光所产生的散射干扰.测定乙酰胆碱和胆碱的线性范围达到l～1000pmol,检测限为500fmol,每次测定时间为2min,寿命为6个月,已用于鼠脑及人血清中乙酰胆碱和胆碱的测定.
Acetylcholinesterase and choline oxidase with molecular recognition function and luminol and Cu ~ (2+) which undergoes the conversion reaction are respectively immobilized in a column of porous glass and ion exchangers to form flow-injection choline and acetylcholine chemistry The luminescence sensor allows the sensor molecular recognition reaction and the transduction reaction to proceed at their optimal pH value.The novel biosensor optimizes the quantum yield of light emission and avoids the scattering interference caused by the direct luminescence on the sensing element.Determination of acetylcholine And choline linear range of l ~ 1000pmol, detection limit of 500fmol, each time for the determination of 2min, life expectancy of 6 months, has been used for the determination of acetylcholine and choline in rat brain and human serum.