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以乙醇为溶剂,以超声波作为提取辅助设备,通过单因子试验确定影响黄酮化合物提取的主要因素及其最佳水平范围,通过L9(34)正交试验确定其最优提取条件,研究超声波辅助提取九香虫黄酮类化合物的最优工艺。最优工艺条件是料液比1:30、提取时间30 min、乙醇浓度70%、提取温度60℃。在最优工艺条件下测得样品中黄酮类化合物的含量达最大,为17.1 mg.g-1。影响超声波辅助提取九香虫黄酮化合物的因素主次为料液比>提取时间>醇浓度>提取温度。
The main factors affecting the extraction of flavonoids and their optimal range were determined by single factor test with ethanol as solvent and ultrasonic as extraction auxiliary. The optimal extraction conditions were determined by orthogonal experiment L9 (34), and the effects of ultrasonic-assisted extraction The optimal technology of flavonoids in nine insects. The optimum conditions were the ratio of material to liquid 1:30, extraction time 30 min, ethanol concentration 70%, extraction temperature 60 ℃. Under the optimum conditions, the content of flavonoids in the sample was the highest, which was 17.1 mg.g-1. Factors influencing the ultrasonic-assisted extraction of nine flavonoids were the ratio of material to liquid> extraction time> alcohol concentration> extraction temperature.