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目的：研究热休克蛋白70（HSP70）在人胃癌组织中的表达。方法：应用抗人HSP70单克隆抗体对190例手术及胃镜活检胃癌及非癌胃粘膜病变石蜡标本，以免疫组织化学ABC法检测HSP70的表达。结果：HSP70在胃癌、慢性浅表性胃炎、肠上皮化生及不典型增生组织中的阳性率分别为73％、23．5％、66％及72％；过表达率分别为56％、5．8％、29．2％及33．33％。HSP70在肠上皮化生、不典型增生及胃癌中的阳性率和过表达率均明显高于单纯性胃炎病变（均P＜0．01）；在胃癌中的过表达率高于在肠上皮化生和不典型增生中（P＜0．05）。HSP70在胃炎中的表达多集中于腺颈部。HSP70阳性染色主要位于胞浆，可见胞核染色。结论：HSP70在人胃癌及癌前病变中过度表达，此一现象可能和胃癌的产生和发展有关。
Objective: To study the expression of heat shock protein 70 (HSP70) in human gastric cancer. METHODS: A total of 190 cases of gastric cancer and non-cancerous gastric mucosa lesions were biopsied by anti-human HSP70 monoclonal antibody. The expression of HSP70 was detected by immunohistochemistry ABC method. Results: The positive rates of HSP70 in gastric cancer, chronic superficial gastritis, intestinal metaplasia, and atypical hyperplasia were 73%, 23.5%, 66%, and 72%, respectively; overexpression rates were 56% and 5 respectively. .8%, 29.2%, and 33.33%. The positive rate and overexpression rate of HSP70 in intestinal metaplasia, atypical hyperplasia and gastric cancer were significantly higher than those of simple gastritis (all P<0.01); the overexpression rate in gastric cancer was higher than that in intestinal epithelialization In birth and dysplasia (P<0.05). The expression of HSP70 in gastritis is mostly concentrated in the neck of the gland. The positive staining of HSP70 was mainly located in the cytoplasm and the nucleus staining was observed. Conclusion: HSP70 is overexpressed in human gastric cancer and precancerous lesions. This phenomenon may be related to the occurrence and development of gastric cancer.