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目的探讨矽肺患者肺泡巨噬细胞(AM)中内质网应激(ERS)凋亡相关基因的表达规律和意义。方法采用单纯随机抽样方法,选择矽肺壹期、贰期和叁期男性患者23、25、24例分别设为矽肺壹期、贰期、叁期组,以8例诊断为观察对象的男性矽尘作业工人为对照组,收集研究对象的支气管肺泡灌洗液,分离、纯化和培养AM后,提取RNA,采用实时定量荧光聚合酶链式反应分析AM中葡萄糖调节蛋白78(GRP78)、CAAT区/增强子结合蛋白同源蛋白(CHOP)、天冬氨酸特异性半胱氨酸蛋白酶(Caspase)-4和Caspase-3的mRNA表达水平。结果矽肺壹期、贰期和叁期组患者AM中GRP78、CHOP、Caspase-4和Caspase-3的mRNA相对表达水平均高于对照组(P<0.05),矽肺贰期组GRP78 mRNA相对表达水平分别高于矽肺壹期和叁期组(P<0.05),矽肺贰期和叁期组患者AM中的CHOP、Caspase-4和Caspase-3的mRNA相对表达水平均高于矽肺壹期组(P<0.05)。多重逐步线性回归分析结果显示,矽肺患者AM中GRP78、CHOP、Caspase-4和Caspase-3的mRNA相对表达水平均与矽肺期别呈正相关(P<0.05)。结论 ERS在矽肺患者AM凋亡的发生与发展中可能发挥重要作用。
Objective To investigate the expression and significance of endoplasmic reticulum stress (ERS) apoptosis-related genes in alveolar macrophages (AM) of patients with silicosis. Methods A simple random sampling method was used to select 23 cases of silicosis stage 1, 2 stage and 3 stage men with 23 cases, 25 cases of 24 cases were set as silicosis stage 1, 2, 3 groups, with 8 cases diagnosed as male silicate The workers were control group, the bronchoalveolar lavage fluid was collected and isolated, purified and cultured. The RNA was extracted and analyzed by real-time quantitative fluorescence polymerase chain reaction (PCR-RFLP) for glucose regulatory protein 78 (GRP78), CAAT / Enhancer mRNA level of CHOP, Caspase-4 and Caspase-3. Results The relative expression levels of GRP78, CHOP, Caspase-4 and Caspase-3 mRNA in AM group were significantly higher than those in control group (P <0.05). The relative expression level of GRP78 mRNA in AMI stage II, (P <0.05). The relative expression levels of mRNA of CHOP, Caspase-4 and Caspase-3 in patients with silicosis II and III were higher than those in silicosis stage I <0.05). Multiple stepwise linear regression analysis showed that the relative expression levels of mRNA of GRP78, CHOP, Caspase-4 and Caspase-3 in AM patients were positively correlated with silicosis (P <0.05). Conclusion ERS may play an important role in the occurrence and development of AM apoptosis in patients with silicosis.