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转录因子可以调节众多下游基因的表达,在植物抗逆境中起重要的调节作用。以南方型紫花苜蓿Medicago sativa‘Millennium’的耐盐突变体为材料,以正常培养(MT_CK2)和盐胁迫(MT_N2)条件下的2个样品叶片进行转录组测序分析,并进行qRT-PCR验证RNA-Seq结果的可靠性,研究耐盐突变体叶片盐胁迫应答相关转录因子基因。结果表明:经过250 mmol/L NaCl胁迫72 h,共检测到30 900个基因表达量发生了变化,7 694个基因差异表达,共有隶属于50个转录家族的422个转录因子发生了差异表达,上调表达268个,下调表达154个。盐胁迫应答基因数量最多的是MYB基因家族,其次是WRKY、NAC、b HLH和AP2-EREBP转录家族。此外,候选出MsANT、Msb HLH36、MsNAI1、Msb ZIP、Msb ZIP73A、MsC3H、MsMYB85、MsNAD、MsMYB、MsNAC、MsTrihelix和MsWRKY等与盐胁迫应答相关的重要转录因子。本研究为揭示紫花苜蓿盐胁迫分子机制奠定基础。
Transcription factors regulate the expression of many downstream genes and play an important regulatory role in plant stress tolerance. Salt tolerant mutants of Medicago sativa’Millennium ’were used as materials, and transcriptome analysis was carried out on two sample leaves under normal conditions (MT_CK2) and salt stress (MT_N2), and qRT-PCR was performed to verify RNA -Seq, and to study the transcription factor genes related to salt stress response in salt tolerant mutants. The results showed that 30 900 genes were detected after 250 mmol / L NaCl stress for 72 h, and 7 694 genes were differentially expressed. There were altogether 422 transcription factors belonging to 50 transcriptional families that were differentially expressed, 268 up-regulated, down-regulated 154. The most abundant salt stress response genes are the MYB gene family, followed by the WRKY, NAC, b HLH and AP2-EREBP transcription families. In addition, important transcription factors related to salt stress responses such as MsANT, Msb HLH36, MsNAI1, Msb ZIP, Msb ZIP73A, MsC3H, MsMYB85, MsNAD, MsMYB, MsNAC, MsTrihelix and MsWRKY are also candidates. This study laid the foundation for revealing the molecular mechanism of salt stress in alfalfa.