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为探讨内源性高甘油三酯血症患者载脂蛋白Ｅ多态性及其与血脂和载脂蛋白水平的关系，采用等电聚焦免疫印迹分析法、酶法及单向免疫扩散法，分别对内源性高甘油三酯血症患者及健康受试者的载脂蛋白Ｅ基因型、表型、空腹血脂及载脂蛋白ＡＩ、Ｂ１００、ＣⅡ、ＣⅢ进行了全面分析。结果发现，与对照组比较，内源性高甘油三酯血症患者空腹血清甘油三酯、总胆固醇、载脂蛋白Ｂ１００、ＣⅡ、ＣⅢ、Ｅ水平明显升高（Ｐ＜０．００１），载脂蛋白ＡＩ明显降低（Ｐ＜０．００１）；对照组与内源性高甘油三酯血症组载脂蛋白Ｅ表型及等位基因频率分布以Ｅ３／３和ε３最高，对照组载脂蛋白Ｅ２亚组血清甘油三酯及载脂蛋白Ｂ１００水平较载脂蛋白Ｅ３或Ｅ４亚组降低（Ｐ＜０．０５），载脂蛋白Ｅ水平升高（Ｐ＜０．０５）；而在内源性高甘油三酯血症组并未发现类似改变（Ｐ＞０．０５）。以上提示，在正常对照组中，ε２可引起低总胆固醇及高载脂蛋白Ｅ水平，从而发挥抗动脉粥样硬化的作用，而内源性高甘油三酯血症患者该保护作用明显下降或消失。
In order to investigate the relationship between apolipoprotein E polymorphism and serum lipids and apolipoprotein levels in patients with endogenous hypertriglyceridemia, isoelectric focusing immunoblot analysis, enzymatic and unidirectional immunodiffusion methods The levels of apolipoprotein E, phenotype, fasting blood lipids and apolipoprotein AI, B100, CⅡ, CⅢ in patients with endogenous hypertriglyceridemia and healthy subjects were analyzed comprehensively. The results showed that the levels of fasting serum triglyceride, total cholesterol, apolipoprotein B100, CⅡ, CⅢ and E in patients with endogenous hypertriglyceridemia were significantly higher than those in the control group (P <0.001) Lipoprotein AI was significantly lower (P <0.001). The phenotype and allele frequencies of apolipoprotein E in control group and endogenous hypertriglyceridemia group were highest with E3 / 3 and ε3, The levels of serum triglyceride and apolipoprotein B100 in E2 subgroup were lower than those in E3 or E4 apolipoprotein E group (P <0.05), and apolipoprotein E level was increased (P <0.05) Hypertriglyceridemia group did not find a similar change (P> 0.05). The above suggests that in the normal control group, ε2 can cause low total cholesterol and high apolipoprotein E levels, thus play a role in anti-atherosclerosis, endogenous hypertriglyceridemia in patients with this protective effect was significantly decreased or disappear.