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为了探索昆虫取食诱导的木本植物体内所产生的防御反应,以合作杨(Populus simonii×P.pyramidalis,‘Opera8277’)扦插苗为实验材料,经杨扇舟蛾(Clostera anachoreta)幼虫取食后,检测叶片中茉莉酸(jasmonate,JA)途径中的关键酶——脂氧合酶(lipoxygenase,LOX)及苯基丙酸类合成途径中的限速酶——苯丙氨酸解氨酶(phenylalanine ammonia lyase,PAL)的活性变化。结果显示,LOX和PAL的活性不仅在虫咬叶片中出现增加,在虫咬叶片上部的系统叶片中也有显著升高,表明茉莉酸途径和苯基丙酸类合成途径被激活,而且防御反应被系统性诱导。并且,与虫咬植株邻近的健康杨树叶片中LOX和PAL的增加表明,杨树间存在由昆虫取食诱导挥发物介导的信息传递。熏蒸实验也证明,茉莉酸甲酯(methyl jasmonate,MeJA)能够作为气体信号诱导合作杨植株产生防御反应。
In order to explore the defensive responses of insect-induced woody plants, the cuttings of Populus simonii × P. pyramidalis (’Opera8277’) were used as experimental materials and fed on larvae of Clostera anachoreta , The key enzyme in the jasmonate (JA) pathway, lipoxygenase (LOX), and the rate-limiting enzyme-phenylalanine ammonia-lyase in phenylpropionate pathway phenylalanine ammonia lyase (PAL) activity changes. The results showed that the activities of LOX and PAL increased not only in the insect bites but also in the leaves of the upper part of the insect bites, indicating that the jasmonate pathway and phenylpropionate pathway were activated, and the defense responses Systematic induction. Also, an increase in LOX and PAL in healthy poplar leaves adjacent to the insect bite plants suggests that there is evidence of volatility-mediated transmission of information by insect feeding between the poplars. Fumigation experiments also demonstrated that methyl jasmonate (MeJA) can act as a gas signaling inducing a defensive response to cooperative poplar plants.