目的 :着重观察鸡胚脾脏凋亡细胞核及其环孔板的形态学变化。方法 :用放线菌酮处理第 15天胚龄鸡胚 ,建立鸡胚脾脏细胞凋亡模型 ,用透射电镜进行观察。结果 :凋亡细胞为各类幼稚血细胞 ,以幼稚淋巴细胞为主 ,巨噬细胞未见凋亡。凋亡细胞核在裂解分离为核碎块的过程中出现环孔板 ,凋亡细胞核完全裂解分离后 ,环孔板消失。结论 :环孔板参与凋亡细胞核的分割以及核碎块的包裹 ,提示凋亡细胞核裂解成为核碎块形成凋亡小体的过程是一个主动的过程。 OBJECTIVE: To observe the morphological changes of apoptotic nucleus and its pericyte in chicken embryo. Methods: Embryo chicken embryos were treated with cycloheximide for 15 days to establish apoptosis model of chick embryo spleen cells. Transmission electron microscopy was used to observe the apoptosis. Results: Apoptotic cells were all kinds of naive blood cells, mainly naive lymphocytes, macrophages no apoptosis. Apoptotic nuclei appear in the process of lysis of the nucleus fragmentation ring plate, apoptotic nuclei completely lysis separation, ring plate disappear. CONCLUSION: Circumference plate is involved in the division of apoptotic nuclei and the inclusion of nuclear fragments, suggesting that nuclear fragmentation of apoptotic nuclei is an active process during the formation of apoptotic bodies by nuclear fragmentation.