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自2002年冬季以来,山东、辽宁等省的养殖刺参几乎每年冬天都大规模爆发溃疡病。本文研究了刺参溃疡病的特征和症状,从病参体表和体内分离出优势菌,经回归感染证实其就是引发本次刺参溃疡病的病原菌。对病原菌进行鉴定发现,该菌革兰氏染色呈阴性,电镜下观察呈短杆状,极端单鞭毛,综合研究该菌在形态、生理生化等方面的特性,确认分离到的病原菌为交替假单胞菌,交替假单胞菌作为养殖刺参的病原菌在国内外属首次报道。
Since the winter of 2002, the culture of sea cucumbers in Shandong and Liaoning provinces has witnessed a large-scale outbreak of ulcer disease every winter. In this paper, the characteristics and symptoms of sea cucumber ulcer disease were studied. The dominant bacteria were isolated from the body surface of the sea cucumber and proved to be the pathogen causing the ulcer disease. Identification of pathogenic bacteria found that the bacteria Gram stain was negative, electron microscopy was short rod-like, extreme single flagella, comprehensive study of the bacteria in the morphological, physiological and biochemical aspects of the characteristics of the isolated pathogenic bacteria confirmed alternate Bacillus, Pseudomonas aeruginosa as breeding pathogenic bacteria in the first report at home and abroad.