在日本药学会第113次年会上,作者已报告了有关鞣质、类黄酮的HPLC定量法。此次运用该法,对日本冰见海滨植物园麻黄栽培品种的栽培条件(肥料中的氮含量、土壤pH)、不同采集时间成分含量的变化进行了探讨,并对麻黄碱类进行了定量。 方法:①将苗分株后栽盆中,每10日施加含氮-磷-钾肥中仅氮含量不同的液体肥料 At the 113th annual meeting of the Japan Pharmaceutical Association, the authors have reported HPLC quantification methods for tannins and flavonoids. The method was used to investigate the changes in the cultivation conditions (nitrogen content in fertilizers, soil pH) and the content of different collection time components of Ephedra cultivars at the Japanese ice-shore Botanical Garden Botanical Garden, and quantification of ephedrines. Method: 1 The seedlings were planted in pots, and every 10 days, nitrogen-phosphorus-potassium fertilizers with only different liquid nitrogen content were applied.