作者把接种临床标本培养16小时的有盖小瓶细胞培养用一种生物素标记的HSVDNA探针试剂盒和单克隆抗体免疫荧光法检测其中的HSV,并与常规试管细胞培养分离病毒作了比较。将可疑为HSV感染者生殖器、口腔、眼和皮肤等部位的标本700×g 40分钟离心,然后接种生长MRC-5单层细胞的常规细胞培养管1支和内置盖片且其上长有单层细胞 The authors cultured 16-hour covered vials of cells that were inoculated with clinical specimens were incubated with a biotin-labeled HSVDNA probe kit and monoclonal antibody immunofluorescence to detect HSV and compared with conventional in vitro culture. Samples of suspicious HSV-infected persons such as genital, oral, eye and skin were centrifuged at 700 × g for 40 minutes and then inoculated with 1 conventional cell culture tube containing MRC-5 monolayer cells and a built-in cover with a single Layer cells