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生殖细胞对环境致害因素十分敏感,观察生殖细胞染色体变化在毒理、药理、遗传、优生等研究中具有实际应用价值。我们参照Tarkowski的方法,加以改良建立了本法。该方法所用设备简单,操作简便,从杀鼠取卵到标本制备完毕不到1h。具体方法介绍如下。①7～9周龄雌性小鼠,腹腔注孕马血清(PMSG、长春生物制品所)10IU/只,48h后注绒毛膜促性腺激素(HCG.上海湖南生化制剂室)20IU/只,
Germ cells are very sensitive to environmental factors, the observation of germ cell chromosome changes in the toxicology, pharmacology, genetics, eugenics and other studies have practical value. We refer to Tarkowski’s method, to be improved to establish this law. The method used in the device is simple and easy to operate. The ovulation from the mouse to the specimen preparation is completed less than 1h. Specific methods are described below. ① Female mice of 7-9 weeks old were injected intraperitoneally with 10 IU / kg of pregnant horse serum (PMSG, Changchun Institute of Biological Products) and 20 IU / kg of chorionic gonadotropin (HCG)