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目的:研究甘草减轻黄药子肝损伤对CYP450酶活性及基因表达的影响。方法:采用紫外分光光度法测定大鼠肝微粒体细胞色素P450、细胞色素b5含量及氨基比林N-脱甲基酶、红霉素N-脱甲基酶活性,采用RT-PCR技术检测大鼠肝脏CYP2E1、CYP3A4的mRNA的表达。结果:黄药子甘草1∶2配伍组能显著减轻黄药子致大鼠肝损害,与黄药子甘草1∶2配伍组(27 g/kg)比较,黄药子组(9 g/kg)肝微粒体蛋白浓度、P450含量、b5含量均明显下降;氨基比林N-脱甲基酶活性、红霉素N-脱甲基酶活性亦明显下降。在mRNA水平上,黄药子组9 g/kg显著诱导CYP2E1、CYP3A4基因的表达,与甘草1∶2配伍后(27 g/kg)表达显著降低。结论:甘草可能通过提高CYP450酶活性,抑制CYP2E1、CYP3A4的mRNA表达,对黄药子致肝损伤具有一定的保护作用。
Objective: To study the effects of Glycyrrhiza uralensis on CYP450 activity and gene expression in liver injury induced by Xanthium. Methods: The contents of cytochrome P450, cytochrome b5 and aminopyrine N-demethylase and erythromycin N-demethylase in rat liver microsome were determined by ultraviolet spectrophotometry. The mRNA expression of CYP2E1 and CYP3A4 in rat liver. Results: Compared with Radix Glycyrrhizae with Radix Glycyrrhizae in the 1: 2 compatibility group (27 g / kg), the Radix Xanthium licorice 1: 2 compatibility group could significantly reduce the liver damage induced by Radix Astragali. The liver microsomal protein concentration in Xanthium subtilis group (9 g / kg) Content and b5 content were significantly decreased; aminopyrine N-demethylase activity, erythromycin N-demethylase activity was also significantly decreased. At the mRNA level, the expression of CYP2E1 and CYP3A4 genes induced by xanthan gum at 9 g / kg was significantly decreased when treated with licorice root 1: 2 (27 g / kg). Conclusion: Glycyrrhiza uralensis may protect CYP450-induced liver injury by increasing the activity of CYP450 and inhibiting the mRNA expression of CYP2E1 and CYP3A4.