论文部分内容阅读
用反相高效液相色谱（ＲＰ－ ＨＰＬＣ） 分析方法对常用中药桔梗中主要有效成分桔梗皂甙Ｄ（ｐｌａｔｙｃｏｄｉｎＤ） 进行了研究，考察了流动相对分离的影响，选择以Ｃ１８ 为色谱分析柱，乙腈－ 磷酸盐缓冲液为流动相，检测波长为２１０ ｎｍ ，进样量在２－０ ～３２－０ μｇ 之间呈良好线性关系，平均回收率为９８－１３ ％ ，ＲＳＤ 为２－３ ％ 。
The effect of mobile phase on the separation of platycodin D, the main active component of common Chinese traditional medicine, was studied by reversed-phase high-performance liquid chromatography (RP-HPLC). C18 chromatographic column, acetonitrile- Phosphate buffer as the mobile phase, the detection wavelength was 210 nm, the injection volume of 2-0 ~ 32-0 μg showed a good linear relationship between the average recovery of 98-13%, RSD of 2-3%.