目的采用siRNA干扰技术沉默人非霍奇金淋巴瘤Raji细胞中Pokemon基因,观察Raji细胞增殖活性的变化,并探讨其可能的分子机制。方法构建靶向Pokemon基因的siRNA重组慢病毒载体并转染Raji细胞。采用实时定量PCR法和Western blotting法检测Raji细胞Pokemon基因的沉默效果,在Raji细胞中沉默Pokemon基因的表达后采用实时定量PCR法和Western blotting法检测bcl-6和突变型p53表达水平的变化;采用流式细胞术检测Pokemon基因沉默后Raji细胞凋亡的情况。结果利用Pokemon靶向siRNA重组慢病毒载体感染Raji细胞有效沉默Raji细胞Pokemon基因表达(P<0.05)。沉默Pokemon基因后,bcl-6和突变型p53基因和蛋白表达均显著降低(P<0.05),细胞凋亡率增高(P<0.05)。结论 siRNA沉默Pokemon基因有效降低了人非霍奇金淋巴瘤Raji细胞的bcl-6和突变型p53基因和蛋白表达,促进细胞凋亡,抑制其增殖。Pokemon基因有望成为非霍奇金淋巴瘤治疗的新靶点。 Objective To silence the Pokemon gene in human non-Hodgkin’s lymphoma Raji cells by siRNA interference and to observe the changes of Raji cell proliferation activity and to explore the possible molecular mechanism. Methods Recombinant lentiviral vector targeting Pokemon gene was constructed and transfected into Raji cells. Real-time PCR and Western blotting were used to detect the silencing effect of Pokemon gene in Raji cells. The expression of Pokemon gene was silenced in Raji cells. The expression of bcl-6 and mutant p53 was detected by real-time quantitative PCR and Western blotting. Flow cytometry was used to detect the apoptosis of Raji cells after Pokemon gene silencing. Results Pokemon targeting siRNA recombinant lentiviral vector infected Raji cells effectively silence Pokemon gene expression in Raji cells (P <0.05). After silence of Pokemon gene, the expression of bcl-6 and mutant p53 gene and protein were significantly decreased (P <0.05), and the apoptosis rate was increased (P <0.05). Conclusion siRNA silencing of Pokemon gene can effectively reduce the expression of bcl-6 and mutant p53 gene and protein in human non-Hodgkin’s lymphoma Raji cells, promote cell apoptosis and inhibit their proliferation. Pokemon gene is expected to become a new target for the treatment of non-Hodgkin’s lymphoma.