论文部分内容阅读
目的探讨阿魏酸钠(SF)对大鼠急性肠系膜静脉血栓(MVT)肠道保护作用及其可能的作用机制。方法 96只雄性SD大鼠随机分为三组,每组32只。A组为模型对照组,造模成功后术后开始皮下注射生理盐水2ml/kg,每12h注射一次,直至术后72h。B组为单纯低分子肝素治疗组,造模成功后术后开始皮下注射低分子肝素300IU/kg,每12h注射1次,直至术后72h。C组为阿魏酸钠+低分子肝素联合治疗组,造模成功后术后开始皮下注射低分子肝素300IU/kg,每12h注射1次,同时给予阿魏酸钠30mg/kg经尾静脉注射,每24h注射一次,直至术后72h。结扎回肠肠系膜上静脉及边缘静脉弓建立MVT周围模型,分别于术后8h、24h、48h及72h采集下腔静脉血及2份回肠组织,检测每组不同时间段血清中血栓烷素B2(TXB2)、血浆D-二聚体(D-Dimer)及肠道组织匀浆液中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力,光镜下观察各时间点肠道组织HE染色做出病理学损伤评分。结果与A组及B组相比,C组血清中TXB2、血浆D-Dimer、肠道组织匀浆中MDA水平均明显降低,SOD水平明显升高,肠道组织病理学损伤评分在24h后均明显降低,差异具有统计学意义(P<0.05)。结论 SF对大鼠MVT模型的治疗具有明显的效果,SF对肠道保护作用的机制与抑制花生四烯酸过度分解代谢,上调局部肠道超氧化物歧化酶活性,抑制脂质过氧化反应有关。
Objective To investigate the intestinal protective effect of sodium ferulate (SF) on acute mesenteric vein thrombosis (MVT) in rats and its possible mechanism. Methods 96 male SD rats were randomly divided into three groups of 32 rats. Group A was model control group. Subcutaneous injection of saline (2ml / kg) was started subcutaneously after successful modeling, and was injected every 12h until 72h after operation. Group B was treated with low molecular weight heparin alone. Subcutaneous injection of low molecular weight heparin (300IU / kg) was given subcutaneously after the successful operation. Injection was given every 12 hours until 72 hours after operation. Group C was sodium ferulate + low molecular weight heparin combined treatment group. Subcutaneous injection of low molecular weight heparin (300IU / kg) was started subcutaneously after the model was established. Injection was given every 12h and sodium ferulate 30mg / kg , Injected once every 24h until 72h after operation. The model of MVT was established by ligating the ileal superior mesenteric vein and the marginal venous arch. The inferior vena cava and the ileal tissue were collected at 8h, 24h, 48h and 72h after operation respectively. The levels of thromboxane B2 (TXB2) ), Plasma D-dimer (D-Dimer) and intestinal homogenate, MDA and SOD activity were observed under light microscope. HE staining of intestinal tissue Make a pathological injury score. Results Compared with group A and group B, the serum levels of TXB2, plasma D-Dimer and intestinal homogenate of group C were significantly decreased, while the level of SOD was significantly increased Was significantly lower, the difference was statistically significant (P <0.05). Conclusion SF has a significant effect on the treatment of MVT model in rats. The mechanism of SF on intestinal protection is related to inhibition of arachidonic acid over-catabolism, up-regulation of local intestinal superoxide dismutase activity and inhibition of lipid peroxidation .