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目的:观察利多卡因(lidocaine)对人体中性粒细胞(PMN)NF-kB的激活和PMN凋亡的影响.方法:将分离的人体PMN分为以下几个给药组:生理盐水对照组;TNF-α组(200 ng/L);TNF-α+lidocaine组(1.0 mmol/L);TNF-α+lidocaine组(2.0 mmol/L);TNF-α +lidocaine组(4.0 mmol/L)共同孵育3 h,用免疫蛋白印迹法观察利多卡因对NF-kB mRNA及其抑制因子(I-kB)mRNA表达,PCR法观察对蛋白含量的影响,以及流式细胞仪方法观察孵育12、24 h后对PMN凋亡的影响.结果:利多卡因组NF-kB mRNA表达显著降低,而I-kB mRNA表达显著增加;并且利多卡因2.0 mmol/L组和4.0 mmol/L组显著优于1.0 mmol/L组(P<0.05),而2.0 mmol/L组和4.0 mmol/L组之间差异无统计学意义.利多卡因在12、24 h(P<0.05)都可以显著抑制TNF-α诱导的PMN凋亡,而4.0 mmol/L组显著优于1.0 mmol/L组抗凋亡效果(P<0.05).结论:利多卡因1.0、2.0、4.0 mmol/L都可以显著下调TNF-α诱导的PMN p65 mRNA的表达,并且在12、24 h可以部分逆转TNF-α诱导的PMN凋亡.“,”AIM:To observe the effect of lidocaine to the activation of NF-kB and apoptosis in human neutrophils(PMN) in vitro.METHODS:Human PMN were divided into five groups:control group (0.9% sodium chloride);TNF-α(200 ng/L);TNF-α + lidocaine (1.0 mmol/L);TNF-α + lidocaine (2.0 mmol/L),and TNF-α + lidocaine (4.0 mmol/L).The expression of NF-kB subunit p65 mRNA in the nuclear and I-kB mRNA in the cytosol extracts were analyzed by Western blotting,after co-incubation for 3 h and the content of p65 protein were observed by RT-PCR.PMN apoptosis was detected on flow cytometry after coincubation 12 h and 24 h.RESULTS:The expression of NF-kB mRNA was significantly decreased and the expression of I-kB mRNA was markedly increased in lidocaine groups (P＜0.05).The expression of p65 was significantly better in lidocaine (2.0 mmol/L),and lidocaine (4.0 mmol/L)than that in lidocaine (1.0 mmol/L).There was no significant difference between lidocaine (2.0 mmol/L) and lidocaine (4.0mmol/L).The PMN apoptosis induced by TNF-α was significant inhibited by dolicaine at 12 h and 24 h(P＜0.05),and lidocaine (4.0 mmol/L) significant was better than that lidocaine (1.0 mmol/L) (P＜0.05).CONCLUSION:Lidocaine (1.0,2.0 or 4.0 mmol/L)can down-regulate the expression of NF-kB subunit p65 mRNA induced by TNF-αand the content of p65 protein in human PMN,and lidocaine can significant reverse the PMN apoptosis induced by TNF-α.