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妊娠中的胚胎类似同种异体移植,但结局却迥然不同。其主要原因可能由于以胎盘滋养叶细胞与母体脱膜间的复杂免疫调节环路。本文报道的正常生理妊娠及正常生育妇女体内的封闭抗体可能在母胎免疫调节中发挥重要作用。 材料和方法 一、研究对象:①正常妊娠组:无流产史的中孕妇女;②正常生育组:1～2年内有足月分娩史的生育期妇女。 二、实验方法:①补体依赖的微量细胞毒试验:参照McIntyre报道的研究方法加以改良,用男方外周血淋巴细胞检测女方血清中的细胞毒抗体,②单向混合淋巴细胞反应封闭试验:参照Takakuwa等研究方法加以改良,将男方外周血淋巴细胞用丝裂霉素处理,分别在12.5％AB型血清中或在12.5％女方血清中与女方外周血淋巴细胞,置于
Embryos in pregnancy are similar to allografts, but the outcome is quite different. The main reason may be due to placental trophoblast cells and maternal desensitization between the complex immune regulatory loop. This article reports the normal physiological pregnancy and normal reproductive women in vivo antibodies may play an important role in immune regulation of the fetus. Materials and Methods First, the research object: ① normal pregnancy group: no history of miscarriage in pregnant women; ② normal fertility group: 1 to 2 years of full-term delivery history of fertility women. Second, the experimental methods: (1) complement-dependent microcytotoxicity test: McIntyre reported research methods to be improved, with the male peripheral blood lymphocytes detection of female serum cytotoxic antibodies, unilateral mixed lymphocyte reaction blocking test: refer to Takakuwa And other research methods to be improved, the male peripheral blood lymphocytes with mitomycin treatment, respectively, in 12.5% AB serum or 12.5% of the female serum with the woman’s peripheral blood lymphocytes, placed