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目的:研究NOD样受体蛋白3(NLRP3)、白细胞介素-6(IL-6)在大鼠心肌缺血再灌注(IR)损伤模型中的表达变化。方法:选择雄性SD大鼠60只,设置IR组30只和假手术组30只。IR组在呼吸机辅助呼吸的前提下,开胸挤出心脏,结扎前降支主干1h,再灌注1h,建立大鼠IR模型。假手术组穿线但不结扎前降支主干,余操作同IR组。造模成功后将心脏组织进行免疫组织化学检测NLRP3的含量,ELISA检测心肌IL-6表达情况。结果:IR组NLRP3较假手术组明显升高,差异有统计学意义(t=55.138,P<0.05);IR组IL-6样本浓度较假手术组明显升高,差异有统计学意义(t=68.106,P<0.05);对于2个变量进行线性相关分析,差异有统计学意义(R=0.989,P<0.05),两者存在正相关性。结论:大鼠心肌IR损伤后NLRP3炎性体表达增加,激活先天免疫系统,促进IL-6的表达增多。
Objective: To investigate the expression of NOD-like receptor 3 (NLRP3) and interleukin-6 (IL-6) in the rat model of myocardial ischemia-reperfusion (IR) injury. Methods: Sixty male Sprague Dawley rats were randomly divided into 30 rats in the IR group and 30 rats in the sham operation group. IR group under the premise of ventilator-assisted breathing, heart thoracotomy, ligation of the descending branch of the trunk 1h, reperfusion 1h, the establishment of rat IR model. Sham-operated group, but not before threading ligation descending trunk, the remaining operation with the IR group. After successful modeling, immunohistochemistry was used to detect the content of NLRP3 in the heart tissue. The expression of IL-6 in myocardium was detected by ELISA. Results: The level of ILRP3 in IR group was significantly higher than that in sham operation group (t = 55.138, P <0.05). The concentration of IL-6 in IR group was significantly higher than that in sham operation group = 68.106, P <0.05). There was a significant positive correlation between the two variables (R = 0.989, P <0.05) for the linear correlation analysis. CONCLUSION: The expression of NLRP3 inflammasome increased after myocardial IR injury in rats, activating the innate immune system and promoting the expression of IL-6.