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舞毒蛾是国际上重要的检疫性农林害虫。本研究采用7对微卫星引物对来自内蒙古赤峰市克什克腾旗、河北茅荆坝、吉林长春、河北大仓、新疆阿勒泰市阿苇滩等我国5个重要舞毒蛾地理种群进行了遗传多样性及遗传分化分析。结果显示,所检测的我国5个舞毒蛾地理种群多数遗传位点显示出纯合子缺失和杂合子过剩的特点。同时进化分析结果显示,所研究的5个舞毒蛾种群可分为两大支,其中新疆阿勒泰种群单独为一支,其余种群聚类为另一支。微卫星遗传多态性分析结果说明,所研究的舞毒蛾种群的个体之间发生了广泛的基因流动。舞毒蛾种群间存在活跃的基因流动,提示该物种在我国一直在传播扩散。该研究可为舞毒蛾的检验检疫及其防控提供依据。
Gypsy moths are internationally important quarantine agroforestry pests. In this study, seven pairs of microsatellite primers were used for genetic diversity of five major Gypsy moths in China, including Keshiketengqi in Chifeng, Hebei Province, Maojingba in Hebei Province, Changchun in Jilin Province, Taikang in Hebei Province and Aishu Beach in Altay City in Xinjiang. Sexual and genetic differentiation analysis. The results showed that the majority of genetic loci detected in the five Gypsy moths in China showed the loss of homozygotes and the excess of heterozygotes. At the same time, phylogenetic analysis showed that the five Gypsy moth populations were divided into two groups, of which Altay and Xinjiang were clustered into the other group. Microsatellite genetic polymorphism analysis showed that a wide range of gene flow occurred among the individuals of the Gypsy moth population. The presence of active gene flow among Gypsy moth populations suggests that the species has been spreading and spreading in our country. This study can provide the basis for the inspection and quarantine of Gypsy moth and its prevention and control.