目的 探讨辛伐他汀对牙槽骨成骨细胞的影响.方法 本实验将牙槽骨成骨细胞分为对照组和低、中、高3个剂量实验组,分别为予以0,1,10,100nmol·L-1辛伐他汀,孵育72 h.用噻唑蓝(MTT)法测定辛伐他汀干预24,48,72 h后的细胞增殖活力,用流式细胞仪法测定辛伐他汀干预后24 h细胞凋亡情况,用实时荧光聚合酶链式反应检测Smad 3 mRNA的表达水平,用酶联免疫吸附实验法检测转化生长因子β1(TGF-β1)的表达变化.结果 干预48,72 h后,中剂量实验组的细胞存活率分别为(69. 10±2. 91)％和(83. 90±3. 56)％,均显著高于对照组的(52. 00±3. 63)％和(70. 00±2. 61)％,差异均有统计学意义(P <0. 05).干预24 h后,低、中、高3个剂量实验组和对照组的细胞凋亡率分别为(17. 10±1. 10)％,(18. 90±1. 22)％,(27. 23±1. 14)％和(28. 70±1. 53)％,低、中剂量实验组的细胞凋亡率和对照组比较,差异均有统计学意义(均P <0. 05).干预24 h后,低、中、高3个剂量实验组和对照组的Smad 3 mRNA分别为(2. 25±0. 46),(1. 95±0. 37),(1. 75±0. 31)和(2. 73±0. 37),低、中、高3个剂量实验组的Smad 3 mRNA和对照组比较,差异均有统计学意义(均P <0. 05).干预24 h后,低、中、高3个剂量实验组和对照组的TGF-β1表达量分别为(43. 42±2. 12),(40. 16±2. 51),(34. 24±3. 13)和(43. 57±3. 82),中、高2个剂量实验组和对照组比较,差异均有统计学意义(均P <0. 05).结论 辛伐他汀通过调节TGF-β1/Smad 3通路抑制牙槽骨成骨细胞的凋亡.“,”Objective To investigate the effect of simvastatin on alveolar osteoblasts. Methods The alveolar osteoblasts were divided into control group and low, medium, high dose experimental groups, which were treated with 0, 1, 10, 100 nmol·L-1 of simvastatin for 72 hours, respectively. At 24, 48, 72 hours after intervention of simvastatin, the cell proliferation activity was measured by methyl thiazolyl tetrazolium (MTT) assay; at 24 hours after intervention of simvastatin, the apoptosis of alveolar osteoblast was detected by flow cytometry; the expression level of Smad 3 mRNA was measured by real-time fluorescent polymerase chain reaction; the expression of transforming growth factor-beta 1 (TGF-β1) was detected by enzyme-linked immunosorbent assay.Results At 48 and 72 hours after intervention, the cell viabilities ofmedium dose experimental group were (69. 10 ± 2. 91) ％ and (83. 90 ± 3. 56) ％, which were significantly higher than those of control group (52. 00 ± 3. 63) ％ and (70. 00 ± 2. 61) ％, the differences were statistically significant between two groups (all P < 0. 05) . At 24 hours after intervention, the apoptotic rates of low, medium, high dose experimental groups and control group were (17. 10 ± 1. 10) ％, (18. 90 ± 1. 22) ％, (27. 23 ± 1. 14) ％ and (28. 70 ± 1. 53) ％, the differences were statistically significant between low-and medium dose experimental groups with control group (all P < 0. 05) . At 24 hours after intervention, the Smad 3 mRNA of low, medium and high dose experimental groups and control group were (2. 25 ± 0. 46) , (1. 95 ± 0. 37) , (1. 75 ± 0. 31) and (2. 73 ± 0. 37) , the differences were statistically significant between low, medium and high dose experimental groups with control group (all P < 0. 05) . At 24 hours after intervention, the expression levels of TGF-β1 in the low, medium and high doses of the experimental groups and control group were (43. 42 ± 2. 12) , (40. 16 ± 2. 51) , (34. 24 ± 3. 13) and (43. 57 ± 3. 82) , the differences were statistically significant between medium and high medium dose experimental groups with control group (all P < 0. 05) . Conclusion Simvastatin can inhibite the apoptosis of alveolar osteoblasts by regulating the activation of transforming growth factor-beta 1/Smad 3 pathway