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甘油三酯(TAG)不但是禽蛋脂质重要的营养组分而且影响禽蛋的贮藏及加工性能。研究利用HPLCMS/MS技术在大气压化学电离(ACPI)模式下完成了鸡蛋、鸭蛋及鹌鹑蛋中TAG分子结构的鉴定及含量分析。研究结果表明:三种禽蛋TAGs中共有35种TAG分子类型,其中OPO(1-油酸-2-棕榈酸-3-油酸)含量最高,其在鸡蛋、鸭蛋和鹌鹑蛋中含量在34.52%~36.86%之间;甘油三酯酰基分布分析表明禽蛋TAGs总碳原子数(CN)在48~56之间,其中CN52含量最高而CN56和CN48的含量则低于1%;禽蛋TAGs的Barcode分析表明三种禽蛋TAGs分子既有相似之处又有相异之处。
Triglyceride (TAG) is not only an important nutritional component of avian egg lipids but also affect the storage and processing performance of avian eggs. The molecular structure of TAG in eggs, duck eggs and quail eggs was identified and analyzed by HPLCMS / MS in atmospheric pressure chemical ionization (ACPI) mode. The results showed that TAGs of three kinds of avian eggs had 35 types of TAGs, of which OPO (1-oleic acid-2-palmitoleic acid-3-oleic acid) had the highest content and its content in eggs, duck eggs and quail eggs was 34.52 % ~ 36.86%. The analysis of triglyceride acyl distribution showed that the total carbon number (CN) of TAGs in eggs was between 48 and 56, with the highest content of CN52 and the content of CN56 and CN48 less than 1% Barcode analysis of the three avian TAGs showed similarities and differences.