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目的:探讨脂蛋白脂酶基因HindIII(rs320)和PvuII(rs285)位点多态与中国人2型糖尿病的相关性。方法:采用聚合酶链反应-限制性片段长度多态性方法,对919个湖北地区汉人(包括2型糖尿病患者481人,健康对照438人)脂蛋白脂酶基因内含子6PvuII和内含子8HindIII位点多态进行基因分型和关联分析,同时对中国大陆人群的相关研究进行meta分析。结果:HindIII和PvuII位点湖北汉族2型糖尿病人和正常人的基因型和等位基因频率均无显著差异。5个研究包括2型糖尿病患者1252例,健康对照1075例的PvuII位点meta分析表明该位点与中国人2型糖尿病无显著相关性(P=0.39);9个研究包括2型糖尿病患者1515例,健康对照1022例的HindIII位点meta分析表明该位点与中国人2型糖尿病也无相关性(P=0.14)。结论:脂蛋白脂酶基因HindIII和PvuII位点多态与中国人2型糖尿病的发生无关。
Objective: To investigate the association between the polymorphisms of HindIII (rs320) and PvuII (rs285) and the type 2 diabetes mellitus in Chinese. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphisms of lipoprotein lipase gene intron 6PvuII and intron of 919 Han Chinese patients (including 481 type 2 diabetic patients and 438 healthy controls) 8HindIII site polymorphism genotyping and association analysis, at the same time on mainland China population meta-analysis of relevant research. Results: There was no significant difference in the genotype and allele frequencies between HindIII and PvuII loci in type 2 diabetics and normal controls in Hubei Han population. Five studies included 1252 patients with type 2 diabetes, and a meta-analysis of PvuII locus in 1075 healthy controls showed no significant association between this locus and type 2 diabetes in Chinese (P = 0.39); 9 studies included 1515 in patients with type 2 diabetes For example, HindIII meta-analysis of 1022 healthy controls showed that there was no association between the locus and type 2 diabetes in Chinese (P = 0.14). Conclusion: HindIII and PvuII polymorphisms of lipoprotein lipase gene are not associated with the development of type 2 diabetes in Chinese.