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目的建立野生和栽培夏枯草的HPLC指纹图谱,为夏枯草药材的鉴别和质量控制提供依据。方法采用高效液相色谱法对28批不同产地的野生和栽培药材进行测定,运用主成分分析和最小偏二乘法-判别分析对其进行模式识别研究,分类并筛选其主要差异性组分。结果建立了野生与栽培夏枯草的HPLC指纹图谱,得到12个共有峰,各产地指纹图谱相似度较高。主成分分析不能完全区分野生与栽培夏枯草,最小偏二乘法-判别分析能进行明确的区分,主要导致差异的成分有6个,其中4个为芦丁、迷迭香酸、咖啡酸和木犀草素。结论指纹图谱与模式识别方法相结合可以区分野生和栽培夏枯草,可以作为夏枯草的质量控制和评价的有效手段之一。
OBJECTIVE To establish HPLC fingerprint of wild and cultivated Prunella vulgaris and provide the basis for the identification and quality control of Prunella medicinal materials. Methods 28 batches of wild and cultivated medicinal herbs in different producing areas were determined by HPLC. The principal component analysis and the least square multiplication-discriminant analysis were used to study the pattern recognition of the wild and cultivated herbs. The main differences were identified and screened. Results The HPLC fingerprinting of wild and cultivated Prunella vulgaris was established and 12 common peaks were obtained. The similarity of fingerprints of each producing area was high. Principal component analysis can not completely distinguish between wild and cultivated Prunella vulgaris, the least square method - discriminant analysis can make a clear distinction, mainly resulting in six different components, of which four are rutin, rosmarinic acid, caffeic acid and luteolin Hormone. Conclusion The combination of fingerprinting and pattern recognition can distinguish wild and cultivated Prunella vulgaris, which can be used as an effective measure for the quality control and evaluation of Prunella vulgaris.