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本文从10例骨肉瘤患者手术切除的实体瘤中,用酶消化和不连续密度梯度离心法分离肿瘤浸润淋巴细胞(TIL),从患者外周血分离淋巴细胞,以4h~(51)Cr 释放试验测定rIL_(-2)(500～100u/ml)激活后的TIL 和LAK 细胞体外抗瘤活性及特异性。用OKT 单克隆抗体对TIL 表型进行分析,结果表明:TIL 和LAK 细胞在培养期间具有良好的抗增速度,培养20d 分别扩增到原来的30～220倍和20～290倍。10例骨肉瘤患者的TIL 培养15～20d 对K_(562)和LiBr 靶细胞(效靶比25∶1)平均杀伤活性分别为56.3%和42.2%,LAK 细胞对K_(562)和LiBr 的杀伤活性分别为
In this study, Tumor infiltrating lymphocytes (TILs) were isolated from 10 patients with osteosarcoma surgically resected. TILs were isolated by enzyme digestion and discontinuous density gradient centrifugation. Lymphocytes were isolated from peripheral blood of patients with 4h ~ (51) Cr release test The antitumor activity and specificity of TIL and LAK cells activated by rIL _ (- 2) (500 ~ 100u / ml) were determined in vitro. TIL phenotype was analyzed by OKT monoclonal antibody. The results showed that TIL and LAK cells had good anti-proliferative rate during culture, and were expanded to 30-220 times and 20-290 times respectively after cultured for 20 days. The killing activity of LAK cells to K_ (562) and LiBr was 56.3% and 42.2%, respectively. The average killing activity of KIL 562 and LiBr target cells (effective target ratio 25: 1) Activity respectively