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定位克隆仍是当前克隆肿瘤易感基因的一条主要途径。关联分析方法和检测患者LOH、纯合性丢失的高发频率区有利于易感基因的定位,削减杂交和脉冲场凝胶电泳的方法有利于目的某因的获取;精细遗传连锁图谱和EST数据库的建立将加速肿瘤易感基因的克隆;从模式生物基因组的研究成果入手来寻找人类肿瘤易感基因是一条可行的途径。
Positioning cloning is still one of the major pathways for cloning cancer susceptibility genes. Association analysis methods and detection of high frequency regions of LOH and loss of homozygosity in patients favor the localization of susceptibility genes, and the methods of reduction hybridization and pulsed field gel electrophoresis are conducive to the acquisition of a certain cause; fine genetic linkage maps and EST database The establishment of clones will speed up the susceptibility of tumor susceptibility genes; the search for human tumor susceptibility genes is a feasible approach from the research results of the model organism genome.