论文部分内容阅读
通过花粉管通道法将人工全序合成的Bt杀虫蛋白基因导入新陆早4号和C6524两品种中,收获注射过Bt杀虫蛋白基因的棉花种子2万余粒。经棉铃虫饲喂的抗虫性生物检测,得到高抗虫的18株新陆早4号转化苗和36株C6524转化苗;以Bt基因的一对引物进行PCR分析,54株抗棉铃虫的转化棉苗均扩增出部分Bt杀虫蛋白基因的目标带。未转化的对照棉苗则无此条带,证实Bt杀虫蛋白基因已整合到转化抗虫棉株的染色体上,首次获得了新疆转目的基因的高效抗虫棉。
The Bt insecticidal protein gene synthesized by artificial sequencing was introduced into Xinluzao 4 and C6524 by pollen tube channel method, and more than 20,000 pieces of cotton seed with Bt insecticidal protein gene were harvested. 18 resistant cultivars of Xinluzao 4 and 36 C6524 transformants were obtained by the insect-resistant bioassay of cotton bollworm. PCR analysis was carried out using a pair of primers of Bt gene. The resistance of 54 cotton bollworm Transformation of cotton seedlings are amplified part of Bt insecticidal protein gene target band. The non-transformed control cotton seedlings had no such bands, which confirmed that the Bt insecticidal protein gene had been integrated into the chromosome of the transformed cotton plant and was the first one to obtain the high-efficiency insect-resistant cotton from Xinjiang.