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目的了解汉族、维吾尔族妇女宫颈癌前病变中不同HPV亚型L1蛋白的表达差异。方法收集HPV阳性的宫颈脱落细胞标本共248例,其中汉族、维吾尔族慢性宫颈炎各48例、46例,CINI各40例、30例,CINII-III各28例、56例。提取HPV DNA进行PCR扩增,采用导流杂交基因芯片技术检测HPV基因型,石蜡切片免疫组化法检测HPV L1蛋白。结果248例患者共检测出15种HPV亚型,随着病变程度的加重,HPV亚型趋于集中,HPV16型检出率最高;维吾尔族HPV16、非HPV16高危亚型中HPV L1蛋白的表达率分别为23.26%(20/86)、43.48%(20/46),差异有统计学意义(P=0.016),HPV16型中汉族与维吾尔族HPV L1蛋白的表达率分别为42.5%(34/80)、23.26%(20/86),差异亦有统计学意义(P=0.008)。结论维吾尔族HPV16型L1蛋白的表达明显低于非HPV16高危亚型,提示HPV L1蛋白的表达缺失可能为宫颈病变中HPV16型感染率最高的原因之一;HPV16型中维吾尔族HPV L1蛋白的表达显著低于汉族,提示HPV L1蛋白的表达缺失可能为维吾尔族宫颈病变患病率高于汉族的原因之一。
Objective To understand the differences of L1 protein expression among different HPV subtypes in cervical precancerous lesions of Han and Uighur women. Methods A total of 248 specimens of cervical exfoliated cells were collected, including 48 cases of chronic cervicitis from Han and Uigur, 46 cases of CINI, 40 cases of CINI, 30 cases of CINI-III and 56 cases of CINII-III. The HPV DNA was extracted for PCR amplification. HPV genotypes were detected by flow cytometry and HPV L1 protein was detected by paraffin section immunohistochemistry. Results A total of 15 HPV subtypes were detected in 248 patients. HPV subtypes tended to be concentrated with the severity of the disease, and the highest detection rate of HPV16 was found. Expression rate of HPV L1 protein in Uighur HPV16 and non-HPV16 high risk subtypes (23.26%, 43.48%, 20/46 respectively), the difference was statistically significant (P = 0.016). The expression rates of HPV L1 protein in Han and Uigur women were 42.5% (34/80) ), 23.26% (20/86), the difference was also statistically significant (P = 0.008). Conclusions The expression of HPV16 L1 protein in Uighur is significantly lower than that in non-HPV16 high-risk subtypes, suggesting that the loss of HPV L1 protein may be one of the causes of HPV16 infection in cervical lesions. HPV L1 protein expression in Uighur HPV16 Which was significantly lower than that in Han nationality, suggesting that the lack of expression of HPV L1 protein may be one of the reasons why the prevalence of cervical lesions in Uyghur people is higher than that in Han people.