作者采用体外试验,研究了淫羊藿Epimedium brevicornum Maxim.叶多酚类提取物的雌激素样活性。该植物叶粉末用异辛烷通氮回流提取,剩余物用甲醇-水(3:1)在氮气下提取,冷冻干燥,得多酚类部位(EB)。该部位经反相HPLC 分离,用水、甲醇-水(1:3)、甲醇-水(1:1)、甲醇-水(3:1)、甲醇连续洗脱,得5个部位 EB-Ⅰ～EB-Ⅴ。用重组酵母检测法和 The authors used in vitro experiments to study the estrogen-like activity of Epimedium brevicornum Maxim. Leaf polyphenols extracts. The plant leaf powder was extracted by refluxing with iso-octane under nitrogen, and the residue was extracted with methanol-water (3:1) under nitrogen, freeze-dried, and much phenolic sites (EB). The fraction was separated by reversed-phase HPLC and successively eluted with water, methanol-water (1:3), methanol-water (1:1), methanol-water (3:1), and methanol to obtain five sites EB-I~ EB-V. Using recombinant yeast assays and