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目的:研究神经型一氧化氮合酶（nNOS）在牙髓牙本质复合体中的表达,探讨其分布与功能的关系。方法:收集新鲜健康人前磨牙60颗,经固定、脱钙、石蜡包埋、切片,nNOS免疫组织化学染色。结果:在根髓nNOS阳性神经纤维呈粗大束状,向颈部逐渐变细,分支少;在颈部,束状纤维呈放射状至冠髓;在冠髓束状纤维大量分支呈网状,部分围绕于血管,部分止于牙髓基质,部分经成牙本质细胞层进入前期牙本质和成熟牙本质近髓1/3牙本质小管。结论:nNOS阳性神经纤维存在于人牙髓牙本质复合体中,部分围绕于血管周围,可能与血管功能调节有关,部分止于牙髓基质、前期本质和牙本质小管内,可能与牙内因素和牙外因素引起的感觉有关。
Objective: To investigate the expression of neuronal nitric oxide synthase (nNOS) in dental pulp dentin complex and its relationship with its distribution and function. Methods: Sixty fresh premolar human teeth were collected, fixed, decalcified, embedded in paraffin, sectioned and stained with nNOS immunohistochemistry. Results: The nNOS-positive nerve fibers in the root nodules showed a large bundle with tapering to the neck with few branches. In the neck, the bundle fibers were radial to the coronal pulp. In the coronal bundle-shaped fibers, many branches were reticular, Around the blood vessels, part of the end of the endodontic matrix, part of the odontoblasts into the pre-dentin and mature dentin near the medulla oblonga dentin tubules. CONCLUSIONS: NNOS-positive nerve fibers exist in the human dental pulp dentin complex, and partially surround the perivascular, which may be related to the regulation of vascular function. Some of the nNOS-positive nerve fibers terminate in the dental pulp matrix, the early nature and the dentinal tubules, And the feeling caused by external factors.