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[目的]观察健骨胶囊含药血清对体外培养新生大鼠成骨细胞增殖和分化的干预作用。[方法]三天给药法制备健骨胶囊含药血清;酶消化法由新生大鼠头盖骨体外分离和培养成骨细胞,应用MTT法测定增殖率及PNPP法测定ALP活性,来评价健骨胶囊对成骨细胞的增殖和分化的作用。[结果]健骨胶囊能提高体外成骨细胞的增殖率、提高ALP活性。[结论]健骨胶囊含药血清对成骨细胞体外增殖与分化具有刺激作用,为健骨胶囊治疗原发性骨质疏松症提供血清药理学依据。
[Objective] To observe the intervention effect of Jiangu capsule containing serum on proliferation and differentiation of cultured newborn rat osteoblasts. [Method] The medicine-containing serum of Jiangu capsule was prepared by three-day administration. The osteoblasts were isolated and cultured from skull of newborn rats by enzymatic digestion method. The proliferation rate was measured by MTT method and the ALP activity by PNPP method. On the proliferation and differentiation of osteoblasts. [Result] Jiangu capsule can increase the proliferation rate of osteoblasts in vitro and increase the activity of ALP. [Conclusion] The Jiangu capsule containing serum can stimulate the proliferation and differentiation of osteoblasts in vitro, and provide the serum pharmacological basis for the treatment of primary osteoporosis by Jiangu capsule.