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目的对采自中国广西省北海附近的块花柳珊瑚(Anthogorgia sp.)进行化学成分的研究。方法用硅胶柱层析(石油醚:丙酮=99:1,49:1,39:1,34:1,29:1,24:1,19:1,14:1,11:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,100%丙酮;氯仿:甲醇=24:1,19:1,14:1,9:1,7:1,5:1,3:1,1:1,1:4,1:9,1:39,100%甲醇)梯度洗脱和SephadexLH-20凝胶柱层析(正己烷:氯仿:甲醇2:1:1洗脱)对块花柳珊瑚丙酮提取物的乙醚部分进行分离纯化,并利用经1HNMR、13CNMR、MS等现代光谱技术对其进行结构鉴定。结果分离得到6个甾醇类化合物以及1个神经酰胺,其结构分别为:麦角甾-5,24(28)-二烯-3β-醇(1)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(2)、(22E,24S)-麦角甾-5,22-二烯-3β-醇(3)、胆甾-5-烯-3β,7β,19α-三醇(4)、(22E)-胆甾-5,22-二烯-3β-醇(5)、胆甾醇(6)、N-正十六碳酰基-正十八碳-4(E)-烯鞘胺醇(7)。结论这些化合物均首次从该种海洋动物中分离得到。
Objective To study the chemical composition of Anthogorgia sp. collected from the North Sea near Guangxi Province, China. Methods Silica gel column chromatography (petroleum ether:acetone=99:1, 49:1, 39:1, 34:1, 29:1, 24:1, 19:1, 14:1, 11:1, 9: 1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,100% acetone; chloroform:methanol=24:1,19:1,14: 1,9:1,7:1,5:1,3:1,1:1,1:4,1:9,1:39,100% methanol gradient elution and Sephadex LH-20 gel column chromatography Alkane:Chloroform:Methanol 2:1:1 elution) The ethyl ether fraction of Aloe argentinosa extract was isolated and purified, and its structure was identified by 1HNMR, 13CNMR, MS and other modern spectroscopic techniques. Results Six sterols and one ceramide were isolated and their structures were as follows: ergot-5, 24(28)-diene-3β-alcohol (1), (22E, 24R)-ergoline-7, 22-diene-3β,5α,6β-triol (2), (22E, 24S)-ergocyan-5,22-diene-3β-ol (3), cholest-5-ene-3β, 7β , 19α-triol (4), (22E)-cholesta-5,22-diene-3β-ol (5), cholesteryl (6), N-hexadecanoyl-n-octadec-4 (E)-Alkyl sphingosine (7). Conclusion These compounds were isolated from this marine animal for the first time.