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中药黄芪和当归均具有行气活血的作用,且两者具有协同作用,体外实验证明其能通过促进内皮细胞增殖来促进血管的生成。本实验用CCK-8法测定黄芪甲苷(AT,黄芪主要有效成分)和阿魏酸(FA,当归主要成分)对体外培养人脐静脉内皮细胞(HUVEc)5 d后的促增殖作用。实验结果表明,当AT和FA总浓度为2μg/mL,且AT和FA比例为7.5∶2.5时能最大限度的促进HUVEc的增殖。
Chinese medicine Astragalus and Angelica are the role of qi and blood circulation, and both have synergistic effects in vitro experiments show that it can promote endothelial cell proliferation to promote blood vessel formation. In this study, CCK-8 method was used to determine the effects of astragaloside (AT, main active ingredient of Astragalus) and ferulic acid (FA, Angelica sinensis) on proliferation of human umbilical vein endothelial cells (HUVEc) for 5 days. The experimental results show that HUVEc proliferation can be maximally promoted when the total concentration of AT and FA is 2μg / mL and the ratio of AT to FA is 7.5: 2.5.