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受污染水体中可能会含有威胁人类身体健康的致病菌。部分致病菌在水中含量少,难分离,而且多变异,所以传统的检测方法难以得到准确的结果。随着分子生物学的发展,很多分子检测技术已经广泛应用到水源致病菌的检测和动态跟踪中。比如荧光定量PCR、环介导等温扩增,以及新发展起来的基因芯片和宏转录组技术等。本文将针对这些分子检测方法以及其他新方法的研究进展进行概述。
Contaminated water may contain pathogenic bacteria that threaten human health. Some pathogenic bacteria in the water content is less difficult to separate, and more variation, so the traditional detection methods difficult to get accurate results. With the development of molecular biology, many molecular detection techniques have been widely applied to the detection and dynamic tracking of waterborne pathogens. Such as fluorescence quantitative PCR, ring-mediated isothermal amplification, and the newly developed gene chip and transcriptome technology. This article will outline the molecular detection methods and other new research methods.