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目的:通过对慢性乙型肝炎患者肝组织及外周血单个核细胞(PBMC)中乙肝病毒共价闭合环状DNA(HBVcccDNA)的特异性定量检测,探讨两者的相关性和临床可行性。方法:取本院慢性乙型肝炎患者肝组织穿刺标本20份,在肝穿刺当天抽取患者外周抗凝血5 mL,标本经相应处理后行HBVcccDNA检测。结果:20份慢性乙型肝炎患者肝组织标本中有18份HBVcccDNA检测为阳性,最大值为5.61×10~5 copy/μg,最小值为3.26×10~3 copy/μg,平均为1.79×10~4 copy/μg。20份血浆中PBMC中HBVcccDNA检测均为阴性。结论:在慢性乙型肝炎患者肝组织中可检测到HBVcccDNA,但未能在PBMC中检测到HBVcccDNA,因此PBMC不能支持HBV的复制。
OBJECTIVE: To investigate the correlation and clinical feasibility of the detection of hepatitis B virus covalently linked circular DNA (HBVcccDNA) in liver tissue and peripheral blood mononuclear cells (PBMCs) from patients with chronic hepatitis B (HBV). Methods: Twenty liver biopsy specimens from patients with chronic hepatitis B in our hospital were collected. On the day of liver biopsy, 5 mL anticoagulant blood was drawn from the peripheral blood of the patients. HBVcc cDNA was detected after corresponding treatment. Results: Twenty HBVcc cDNAs from 20 chronic hepatitis B patients were positive, with a maximum value of 5.61 × 10 ~ 5 copies / μg and a minimum value of 3.26 × 10 ~ 3 copy / μg with an average of 1.79 × 10 ~ 4 copy / μg. 20 copies of HBV DNA in plasma PBMC detection were negative. CONCLUSIONS: HBVcccDNA is detectable in the liver of patients with chronic hepatitis B but HBVcccDNA is not detected in PBMCs, so that PBMCs can not support HBV replication.