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红根草是广西道地药材,丹参酮ⅡA是红根草的主要活性作用成分之一,因此,筛选出丹参酮ⅡA含量高的优良种质,对解决红根草资源紧缺问题及提高其药材质量均有重要作用。本研究利用傅里叶红外光谱技术对不同产地红根草化学成分的红外吸收特征峰进行了测定,利用HPLC技术不同产地红根草中丹参酮ⅡA含量,同时,借助化学计量学方法比较分析了不同产地样品的红外光谱吸收峰与丹参酮ⅡA相关性。结果表明,不同产地红根草化学成分组成比较相似,但化学成分的含量却有较大差异。多元对数相关分析技术显示,1 734 cm~(-1)、1 632 cm~(-1)、1 548 cm~(-1)、1 515 cm~(-1)、830 cm~(-1)及789 cm~(-1)等处附近的吸收强度与实际测定不同产地红根草丹参酮ⅡA含量均有密切关系。两种方法均一致显示,丹参酮ⅡA含量以江西南丰类型最高,其次是广西钟山类型,其中以江西奉新类型含量最低。
Red root grass is a native medicinal material in Guangxi, tanshinone Ⅱ A is one of the main active components of red root grass, therefore, screening high quality tanshinone Ⅱ A germplasm, red root grass to solve the problem of shortage of resources and improve their medicinal quality Have an important role. In this study, Fourier transform infrared spectroscopy (FTIR) was used to determine the infrared absorption peak of chemical constituents of Anthurium andraeanum in different habitats. HPLC method was used to determine the content of tanshinoneⅡA in Anthurium andraeanum. Meanwhile, Correlation of Infrared Spectral Absorption Peak and Tanshinone ⅡA in Origin Samples. The results showed that the composition of chemical constituents of red root grass from different origins was similar, but the content of chemical constituents was quite different. Multivariate logarithmic correlation analysis showed that the correlation coefficient of the correlation coefficients was 1 734 cm -1, 1 632 cm -1, 1 548 cm -1, 1 515 cm -1, 830 cm -1 ) And 789 cm ~ (-1) were closely related to the actual determination of tanshinone Ⅱ A content in different origins. Both methods showed that the content of tanshinone ⅡA was the highest in Nanfeng, Jiangxi Province, followed by the Zhongshan of Guangxi, and the lowest in Fengxin, Jiangxi.