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Aim:To explore the potential of electroporation (EP)-mediated hepatitis B virus(HBV) DNA vaccination for the treatment of chronic HBV infection.Methods:BALB/c mice were vaccinated with HBV DNA vaccine encoding for the HBVpreS_2-S antigen,combined with or without EP.HBV surface antigen expressionplasmid was administered into mice liver via a hydrodynamic injection to mimicHBV infection.The clearance of antigen in the serum and liver was detected byELISA assay and immunohistochemical staining.The histopathology of the livertissues was examined by HE staining and serum alanine aminotransferase assay.Results:The immunogenicity of HBV DNA vaccine encoding for the HBV preS_2-S antigen can be improved by EP-mediated vaccine delivery.The elicited immuneresponses can indeed reduce the expression of HBV surface antigen (HBsAg) inhepatocytes of the mouse model that was transfected to express HBsAg using thehydrodynamic injection method.The antigen clearance process did not causesignificant toxicity to liver tissue,suggesting a non-cytolytic mechanism.Conclusion:The EP-aided DNA vaccination may have potential in mediating viralclearance in chronic hepatitis B patients.
Aim: To explore the potential of electroporation (EP) -mediated hepatitis B virus (HBV) DNA vaccination for the treatment of chronic HBV infection. Methods: BALB / c mice were vaccinated with HBV DNA vaccine encoding for the HBV preS_2-S antigen, combined with or without EP. HBV surface antigen expression plasmids was administered into mice liver via a hydrodynamic injection to mimic HBV. The clearance of antigen in the serum and liver was detected by ELISA assay and immunohistochemical staining. The histopathology of the livertissues was examined by HE staining and Serum alanine aminotransferase assay. Results: The immunogenicity of HBV DNA vaccine encoding for the HBV preS_2-S antigen can be improved by EP-mediated vaccine delivery. The elicited immuneresponses can indeed reduce the expression of HBV surface antigen (HBsAg) inhepatocytes of the mouse model that was transfected to express HBsAg using the hydrodynamic injection method. antigen proofing process did not causeignificant toxici suggesting that a non-cytolytic mechanism. Conlusion: The EP-aided DNA vaccination may have in mediating viral clearance in chronic hepatitis B patients.