【摘 要】
：
目的:开发一种白细胞介素-2(IL-2)长效缓释微球剂型。方法:采用S/O/W法制备了白介素-2因子多糖微粒的PLGA微球,考察了微球的表面形态、粒径分布等,并且运用ELISA方法考察了微
【机 构】
：
上海市第十人民医院,上海交通大学药学院,上海交通大学医学院附属新华医院,深圳赛宝儿生物药业有限公司,
论文部分内容阅读
目的:开发一种白细胞介素-2(IL-2)长效缓释微球剂型。方法:采用S/O/W法制备了白介素-2因子多糖微粒的PLGA微球,考察了微球的表面形态、粒径分布等,并且运用ELISA方法考察了微球的体外释放效果。结果:本方法制备的白介素-2因子微球光滑圆整,粒径分布较均匀,体外缓释达32天,累积释放率近90%。结论:本方法制备的白介素-2因子微球,不仅具有有效地保护IL-2蛋白活性,同时实现长效缓释的目标,是一种可行的蛋白缓释方案。
OBJECTIVE: To develop a long-acting sustained-release microsphere dosage form of interleukin-2 (IL-2). Methods: PLGA microspheres with interleukin-2 factor polysaccharide particles were prepared by S / O / W method. The morphology and particle size distribution of microspheres were investigated. The release of microspheres in vitro was evaluated by ELISA. Results: The interleukin-2 factor microspheres prepared by this method were smooth and round with uniform particle size distribution and sustained release for 32 days in vitro with a cumulative release rate of nearly 90%. CONCLUSION: IL-2-loaded microspheres prepared by this method not only have the potential to effectively protect IL-2 protein and achieve long-term sustained release, it is a viable protein delivery protocol.
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